Proteomics Academy

Protein-protein interactions are one of the main driving forces underlying all cellular processes and the knowledge of protein interfaces is the key to understanding cellular functions. Complementary to the classical methods of structural biology (X-ray crystallography, NMR spectroscopy and electron microscopy), cross-linking combined with mass spectrometry (MS) has developed into an alternative technique to obtain valuable information about the structure and dynamics of macromolecular assemblies in solution.

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In this workshop, fundamentals of the cross-linking MS strategy are introduced. Different cross-linking strategies using non-cleavable and MS-cleavable cross-linkers as well as in vivo cross-linking are presented. Practical aspects of two complementary software tools (MeroX) and on modeling protein complexes based on cross-linking constraints are taught.